Construction of a high-density genetic map with whole genome sequencing in Nicotiana tabacum L.
Genomics
; 112(2): 2028-2033, 2020 03.
Article
em En
| MEDLINE
| ID: mdl-31760041
ABSTRACT
Tobacco (Nicotiana tabacum L.) is an essential commercial crop and an ideal model plant for biological mechanism studies. As an allopolyploid species, tobacco harbors a massive and complex genome, which makes the application of molecular markers complicated and challenging. In our study, we performed whole-genome sequencing of an intraspecific recombinant inbred line (RIL) population, a F1 generation and their parents. With the Nicotiana tabacum (K326 cultivar) genome as reference, a total of 45,081 markers were characterized to construct the genetic map, which spanned a genetic distance of 3486.78 cM. Evaluation of a two-dimensional heat map proved the high quality of the genetic map. We utilized these markers to anchor scaffolds and analyzed the ancestral genome origin of linkage groups (LGs). Furthermore, such a high-density genetic map will be applied for quantitative trait locus (QTL) detection, gene localization, genome-wide association studies (GWAS), and marker-assisted breeding in tobacco.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nicotiana
/
Genoma de Planta
/
Ligação Genética
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Genomics
Assunto da revista:
GENETICA
Ano de publicação:
2020
Tipo de documento:
Article