Your browser doesn't support javascript.
loading
Genome-Wide Transcriptome Analysis of Rice Seedlings after Seed Dressing with Paenibacillus yonginensis DCY84T and Silicon.
Yoo, Yo-Han; Kim, Minjae; Chandran, Anil Kumar Nalini; Hong, Woo-Jong; Ahn, Hye Ryun; Lee, Gang Taik; Kang, Sungju; Suh, Dabin; Kim, Jin-O; Kim, Yeon-Ju; Jung, Ki-Hong.
Afiliação
  • Yoo YH; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.
  • Kim M; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.
  • Chandran AKN; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.
  • Hong WJ; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.
  • Ahn HR; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.
  • Lee GT; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.
  • Kang S; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.
  • Suh D; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.
  • Kim JO; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.
  • Kim YJ; Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Yongin 17104, Korea.
  • Jung KH; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.
Int J Mol Sci ; 20(23)2019 Nov 23.
Article em En | MEDLINE | ID: mdl-31771205
ABSTRACT
Plant-growth-promoting bacteria (PGPB) are beneficial microorganisms that can also protect against disease and environmental stress. Silicon (Si) is the second most abundant element in soil, and is known to increase plant growth, grain yield, resistance to biotic stress, and tolerance to abiotic stress. Combined treatment of PGPB and Si has been shown to further enhance plant growth and crop yield. To determine the global effects of the PGPB and Si on rice growth, we compared rice plants treated with Paenibacillus yonginensis DCY84T (DCY84T) and Si with untreated rice. To identify the genes that respond to DCY84T+Si treatment in rice, we performed an RNA-Seq transcriptome analysis by sampling treated and untreated roots on a weekly basis for three weeks. Overall, 576 genes were upregulated, and 394 genes were downregulated in treated roots, using threshold fold-changes of at least 2 (log2) and p-values < 0.05. Gene ontology analysis showed that phenylpropanoids and the L-phenylalanine metabolic process were prominent in the upregulated genes. In a metabolic overview analysis using the MapMan toolkit, pathways involving phenylpropanoids and ethylene were strongly associated with upregulated genes. The functions of seven upregulated genes were identified as being associated with drought stress through a literature search, and a stress experiment confirmed that plants treated with DCY84T+Si exhibited greater drought tolerance than the untreated control plants. Furthermore, the predicted protein-protein interaction network analysis associated with DCY84T+ Si suggests mechanisms underlying growth promotion and stress tolerance.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oryza / Silício / Plântula Idioma: En Revista: Int J Mol Sci Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oryza / Silício / Plântula Idioma: En Revista: Int J Mol Sci Ano de publicação: 2019 Tipo de documento: Article