An RNAi suppressor activates in planta virus-mediated gene editing.

Zhang, Xian; Kang, Lihua; Zhang, Qi; Meng, Qiqi; Pan, Yafei; Yu, Zhiming; Shi, Nongnong; Jackson, Stephen; Zhang, Xinlian; Wang, Huizhong; Tor, Mahmut; Hong, Yiguo

Zhang, Xian; Kang, Lihua; Zhang, Qi; Meng, Qiqi; Pan, Yafei; Yu, Zhiming; Shi, Nongnong; Jackson, Stephen; Zhang, Xinlian; Wang, Huizhong; Tor, Mahmut; Hong, Yiguo.

Afiliação

Zhang X; Research Centre for Plant RNA Signaling and Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Kang L; Research Centre for Plant RNA Signaling and Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Zhang Q; Research Centre for Plant RNA Signaling and Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Meng Q; Research Centre for Plant RNA Signaling and Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Pan Y; Research Centre for Plant RNA Signaling and Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Yu Z; Research Centre for Plant RNA Signaling and Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Shi N; Research Centre for Plant RNA Signaling and Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Jackson S; Warwick-Hangzhou RNA Signaling Joint Laboratory, School of Life Sciences, University of Warwick, Warwick, CV4 7AL, UK.
Zhang X; Department of Family Medicine and Public Health, Division of Biostatistics & Bioinformatics, University of California San Diego, La Jolla, CA, 92093, USA.
Wang H; Research Centre for Plant RNA Signaling and Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Tor M; Department of Biology, School of Science and the Environment, Worcester-Hangzhou Joint Molecular Plant Health Laboratory, University of Worcester, Worcester, WR2 6AJ, UK.
Hong Y; Research Centre for Plant RNA Signaling and Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China. yiguo.hong@hznu.edu.cn.

Funct Integr Genomics ; 20(4): 471-477, 2020 Jul.

Article em En | MEDLINE | ID: mdl-31848794

RESUMO

RNA-guided CRISPR/Cas9 technology has been developed for gene/genome editing (GE) in organisms across kingdoms. However, in planta delivery of the two core GE components, Cas9 and small guide RNA (sgRNA), often involves time-consuming and labor-intensive production of transgenic plants. Here we show that Foxtail mosaic virus, a monocot- and dicot-infecting potexvirus, can simultaneously express Cas9, sgRNA, and an RNAi suppressor to efficiently induce GE in Nicotiana benthamiana through a transgenic plant-free manner.

Assuntos

Edição de Genes/métodos; Nicotiana/genética; Potexvirus/genética; RNA Interferente Pequeno/genética; Proteína 9 Associada à CRISPR/genética; Proteína 9 Associada à CRISPR/metabolismo; RNA Guia de Cinetoplastídeos/genética; RNA Guia de Cinetoplastídeos/metabolismo; RNA Interferente Pequeno/metabolismo

Palavras-chave

CRISPR/Cas9; FoMV; RNAi suppressor; VmGE

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nicotiana / Potexvirus / RNA Interferente Pequeno / Edição de Genes Idioma: En Revista: Funct Integr Genomics Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China

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