A paper-based biosensor for visual detection of glucose-6-phosphate dehydrogenase from whole blood.
Analyst
; 145(5): 1817-1824, 2020 Mar 02.
Article
em En
| MEDLINE
| ID: mdl-31956872
ABSTRACT
Screening for a deficiency of glucose-6-phosphate dehydrogenase (G6PD) in red blood cells is vital for determining the potentially life-threatening presence of congenital, hereditary or induced hemolytic anemias. In this study, a "sample-to-readout" paper-based point-of-care (POC) colorimetric biosensor was developed for direct detection of G6PD in whole blood by simple visual comparison to a color card. The G6PD paper sensor was highly stable with no observable loss in performance after room temperature storage for at least 6 weeks, and worked equally well at room temperature and 37 °C. The simple printed paper format and the stability of the colorimetric reagents facilitates scalable manufacturing. The ability to utilize well established sample collection and preparation protocols along with a colorimetric visual readout should facilitate future transfer of this proof-of-concept POC biosensor to remote or resource-poor locations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Colorimetria
/
Eritrócitos
/
Glucosefosfato Desidrogenase
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Analyst
Ano de publicação:
2020
Tipo de documento:
Article