Occurrence and development of diabetic nephropathy caused by CD63 by inhibiting Wnt-ß-catenin signaling pathway.
Eur Rev Med Pharmacol Sci
; 24(1): 284-294, 2020 01.
Article
em En
| MEDLINE
| ID: mdl-31957842
ABSTRACT
OBJECTIVE:
This study aimed to investigate the occurrence and development of diabetic nephropathy caused by CD63 by inhibiting Wnt-ß-catenin signaling pathway. PATIENTS ANDMETHODS:
Renal tissues and normal renal tissues distant from renal lesions of patients with diabetic nephropathy treated in The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University from January 2018 to November 2018 were selected. Human renal tubular epithelial cell HKC was purchased. CD63-siRNA group, NC group, blank group, CD63-mimics, CD63-mimics+si-Wnt4, and CD63-inhibitor+sh-Wnt4 were transfected into renal tubular epithelial cell HKC; mRNA expression in the cells was detected by qRT-PCR, and the protein expression in the cells was detected by WB. CCK8 and flow cytometry were used to detect cell proliferation and apoptosis.RESULTS:
CD63, Wnt4, ß-catenin, and p-GSK-3ß were highly expressed in diabetic nephropathy. Cell experiments showed that inhibiting CD63 and Wnt-ß-catenin signaling pathway could promote cell proliferation and reduce cell apoptosis, and the protein expressions of Wnt4, ß-catenin, p-GSK-3ß, and Bcl-2 were significantly reduced. Rescue experiments showed that after the co-transfection of CD63-mimics+si-Wnt4 and CD63-inhibitor+sh-Wnt4 into EC109 and EC9706, the cell proliferation and apoptosis rates were not different from those of the NC group without transfection sequence.CONCLUSIONS:
CD36 can mediate cell apoptosis by inhibiting the expression of the related proteins in nodal Wnt/ß-catenin signaling pathway, and is expected to become a potential therapeutic target for clinical treatment of patients with diabetic nephropathy.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nefropatias Diabéticas
/
Tetraspanina 30
/
Via de Sinalização Wnt
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Eur Rev Med Pharmacol Sci
Assunto da revista:
FARMACOLOGIA
/
TOXICOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article