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Incorporation of Indole Significantly Improves the Transfection Efficiency of Guanidinium-Containing Poly(Methacrylamide)s.
Cokca, Ceren; Zartner, Leon; Tabujew, Ilja; Fischer, Dagmar; Peneva, Kalina.
Afiliação
  • Cokca C; Institute of Organic Chemistry and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Lessingstraße 8, 07743, Jena, Germany.
  • Zartner L; Institute of Pharmacy, Pharmaceutical Technology and Biopharmacy, Friedrich Schiller University Jena, Lessingstraße 8, 07743, Jena, Germany.
  • Tabujew I; Institute of Organic Chemistry and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Lessingstraße 8, 07743, Jena, Germany.
  • Fischer D; Institute of Pharmacy, Pharmaceutical Technology and Biopharmacy, Friedrich Schiller University Jena, Lessingstraße 8, 07743, Jena, Germany.
  • Peneva K; Jena Center of Soft Matter, Friedrich Schiller University Jena, Philosophenweg 7, 07743, Jena, Germany.
Macromol Rapid Commun ; 41(6): e1900668, 2020 Mar.
Article em En | MEDLINE | ID: mdl-32077146
ABSTRACT
A highly efficient transfection agent is reported that is based on terpolymer consisting of N-(2-hydroxypropyl)methacrylamide (HPMA), N-(3-guanidinopropyl) methacrylamide (GPMA), and N-(2-indolethyl)methacrylamide monomers (IEMA) by analogy to the amphipathic cell-penetrating peptides containing tryptophan and arginine residues. The incorporation of the indole-bearing monomer leads to successful plasmid DNA condensation even at a nitrogen-to-phosphate (N/P) ratio of 1. The hydrodynamic diameter of polyplexes is determined to be below 200 nm for all N/P ratios. The transfection studies demonstrate a 200-fold increase of the transgene expression in comparison to P(HPMA-co-GPMA) with the same guanidinium content. This study reveals the strong potential of the indole group as a side-chain pendant group that can increase the cellular uptake of polymers and the transfection efficiency of the respective polyplexes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polímeros / Resinas Acrílicas / Transfecção / Guanidina / Guanidinas / Indóis Limite: Animals Idioma: En Revista: Macromol Rapid Commun Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polímeros / Resinas Acrílicas / Transfecção / Guanidina / Guanidinas / Indóis Limite: Animals Idioma: En Revista: Macromol Rapid Commun Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Alemanha