Efficient generation of zebrafish maternal-zygotic mutants through transplantation of ectopically induced and Cas9/gRNA targeted primordial germ cells.

Zhang, Fenghua; Li, Xianmei; He, Mudan; Ye, Ding; Xiong, Feng; Amin, Golpour; Zhu, Zuoyan; Sun, Yonghua

Zhang, Fenghua; Li, Xianmei; He, Mudan; Ye, Ding; Xiong, Feng; Amin, Golpour; Zhu, Zuoyan; Sun, Yonghua.

Afiliação

Zhang F; State Key Laboratory of Freshwater Ecology and Biotechnology, Innovation Academy for Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
Li X; State Key Laboratory of Freshwater Ecology and Biotechnology, Innovation Academy for Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
He M; State Key Laboratory of Freshwater Ecology and Biotechnology, Innovation Academy for Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
Ye D; State Key Laboratory of Freshwater Ecology and Biotechnology, Innovation Academy for Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
Xiong F; State Key Laboratory of Freshwater Ecology and Biotechnology, Innovation Academy for Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
Amin G; State Key Laboratory of Freshwater Ecology and Biotechnology, Innovation Academy for Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
Zhu Z; State Key Laboratory of Freshwater Ecology and Biotechnology, Innovation Academy for Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
Sun Y; State Key Laboratory of Freshwater Ecology and Biotechnology, Innovation Academy for Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic add

J Genet Genomics ; 47(1): 37-47, 2020 01 20.

Article em En | MEDLINE | ID: mdl-32094061

Assuntos

Sistemas CRISPR-Cas/genética; Genoma/genética; Peixe-Zebra/genética; Zigoto/crescimento & desenvolvimento; Animais; Técnicas de Inativação de Genes; Marcação de Genes; Células Germinativas/crescimento & desenvolvimento; Humanos; Mutação/genética; Fenótipo; RNA Guia de Cinetoplastídeos/genética; Peixe-Zebra/crescimento & desenvolvimento

Palavras-chave

CRISPR/Cas9; Maternal zygotic mutant; Primordial germ cells; Transplantation; Zebrafish

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zigoto / Peixe-Zebra / Genoma / Sistemas CRISPR-Cas Limite: Animals / Humans Idioma: En Revista: J Genet Genomics Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China

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