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Probing Heterogeneity in Bovine Enamel Composition through Nanoscale Chemical Imaging using Atom Probe Tomography.
Licata, Olivia; Guha, Upoma; Poplawsky, Jonathan D; Aich, Nirupam; Mazumder, Baishakhi.
Afiliação
  • Licata O; Department of Materials Design and Innovation, University at Buffalo, The State University of New York, Buffalo, NY 14260, United States.
  • Guha U; Department of Restorative Dental Sciences, University of Florida, Gainesville, FL 32610, United States.
  • Poplawsky JD; Center for Nanophase Materials Science, Oak Ridge National Laboratory, Oak Ridge, TN 37831, United States.
  • Aich N; Department of Civil, Structural and Environmental Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, United States. Electronic address: nirupama@buffalo.edu.
  • Mazumder B; Department of Materials Design and Innovation, University at Buffalo, The State University of New York, Buffalo, NY 14260, United States. Electronic address: baishakh@buffalo.edu.
Arch Oral Biol ; 112: 104682, 2020 Apr.
Article em En | MEDLINE | ID: mdl-32126356
ABSTRACT

OBJECTIVE:

The aim of this study was to determine the heterogeneity in chemical composition of bovine enamel using atom probe tomography, and thereby evaluate the suitability of bovine enamel as a substitute for human enamel in in vitro dental research.

DESIGN:

Enamel samples from extracted bovine incisor teeth were first sectioned using a diamond saw and then milled into needle-like samples (<100 nm diameter) by focused ion beam (FIB) coupled with a scanning electron microscope (SEM). These samples were analyzed in the atom probe to acquire three-dimensional (3D) images and quantify the atomic chemistry and distribution in bovine enamel.

RESULTS:

For the first time, the atomic-level composition and clustering of major constituents and impurities within bovine enamel were determined and imaged. We discovered that the chemical composition of bovine enamel is spatially inhomogeneous at the atomic scale. The average bulk Ca/P ratio, ∼1.4, was in agreement with previously reported literature values from alternative conventional methods. When assessed locally at the atomic scale, the Ca/P ratio varied between 1.1 and 2.03. We also discovered that the Mg impurities were significantly segregated throughout the enamel, and such clustering influenced the variation of Ca/P ratios. The increase in Mg concentrations, near the Mg clusters, correlated with increased Ca and decreased P concentrations.

CONCLUSION:

The presented findings of variability in local composition should be taken into account when interpreting dental research results from bovine enamel.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tomografia / Esmalte Dentário Limite: Animals Idioma: En Revista: Arch Oral Biol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tomografia / Esmalte Dentário Limite: Animals Idioma: En Revista: Arch Oral Biol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos