Microcystin-LR impairs glucose metabolism in pancreatic ß cells in vivo and in vitro.
Toxicol Lett
; 326: 106-113, 2020 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-32142839
ABSTRACT
This study aimed to investigate the toxic effects of microcystin-LR (MC-LR), which is released from several bloom-forming cyanobacteria, on the glucose metabolism of pancreatic ß cells in vivo and in vitro. Male mice and the pancreatic MIN6 cells were respectively treated with varying concentrations of MC-LR. After 3- or 6- months of MC-LR exposure, increase in the body weight of mice was found to be inhibited, and the structure of their pancreatic tissues was damaged with impaired glucose tolerance and impaired insulin secretion. Further, these toxic effects became more pronounced with time and with increased dosages. Direct cytotoxic effects of MC-LR were observed in the MIN6 pancreatic ß-cells possibly due to their expression of the MC-LR specific transporter. MC-LR entered the MIN6 cells that significantly reduced the cell viability. Both in vivo and in vitro experiments demonstrated that MC-LR was able to induce apoptosis, possibly associated with mitochondrial damage. Above all, these findings implied that MC-LR may be transported into the pancreatic ß cells and cause subsequent cytotoxicity.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pâncreas
/
Sobrevivência Celular
/
Cianobactérias
/
Apoptose
/
Células Secretoras de Insulina
/
Microcistinas
/
Glucose
Limite:
Animals
Idioma:
En
Revista:
Toxicol Lett
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China