Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli.

Ding, Tingting; Huang, Chaoyong; Liang, Zeyu; Ma, Xiaoyan; Wang, Ning; Huo, Yi-Xin

Ding, Tingting; Huang, Chaoyong; Liang, Zeyu; Ma, Xiaoyan; Wang, Ning; Huo, Yi-Xin.

Afiliação

Ding T; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Haidian District, Beijing, 100081, China.
Huang C; SIP-UCLA Institute for Technology Advancement, Suzhou, 215123, China.
Liang Z; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Haidian District, Beijing, 100081, China.
Ma X; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Haidian District, Beijing, 100081, China.
Wang N; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Haidian District, Beijing, 100081, China. xyma@bit.edu.cn.
Huo YX; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Haidian District, Beijing, 100081, China. wangning@bit.edu.cn.

Microb Cell Fact ; 19(1): 63, 2020 Mar 10.

Article em En | MEDLINE | ID: mdl-32156270

Assuntos

Clonagem Molecular/métodos; Escherichia coli/genética; Edição de Genes/métodos; Plasmídeos/genética; RNA Guia de Cinetoplastídeos/genética; Recombinação Genética; Deleção de Sequência

Palavras-chave

Direct repeats; Inverted repeats; Large genomic deletion; Paired gRNAs; Plasmid stability

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmídeos / Clonagem Molecular / RNA Guia de Cinetoplastídeos / Escherichia coli / Edição de Genes Idioma: En Revista: Microb Cell Fact Assunto da revista: BIOTECNOLOGIA / MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China

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