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Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.
Matsuyama, Shutoku; Nao, Naganori; Shirato, Kazuya; Kawase, Miyuki; Saito, Shinji; Takayama, Ikuyo; Nagata, Noriyo; Sekizuka, Tsuyoshi; Katoh, Hiroshi; Kato, Fumihiro; Sakata, Masafumi; Tahara, Maino; Kutsuna, Satoshi; Ohmagari, Norio; Kuroda, Makoto; Suzuki, Tadaki; Kageyama, Tsutomu; Takeda, Makoto.
Afiliação
  • Matsuyama S; Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; matuyama@nih.go.jp mtakeda@nih.go.jp.
  • Nao N; Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Shirato K; Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Kawase M; Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Saito S; Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Takayama I; Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Nagata N; Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Sekizuka T; Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Katoh H; Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Kato F; Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Sakata M; Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Tahara M; Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Kutsuna S; Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.
  • Ohmagari N; Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.
  • Kuroda M; Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Suzuki T; Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Kageyama T; Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  • Takeda M; Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; matuyama@nih.go.jp mtakeda@nih.go.jp.
Proc Natl Acad Sci U S A ; 117(13): 7001-7003, 2020 03 31.
Article em En | MEDLINE | ID: mdl-32165541
ABSTRACT
A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia Viral / Serina Endopeptidases / Infecções por Coronavirus / Betacoronavirus Limite: Animals / Humans Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia Viral / Serina Endopeptidases / Infecções por Coronavirus / Betacoronavirus Limite: Animals / Humans Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2020 Tipo de documento: Article