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Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies against SARS-CoV-2.
Liu, Wanbing; Liu, Lei; Kou, Guomei; Zheng, Yaqiong; Ding, Yinjuan; Ni, Wenxu; Wang, Qiongshu; Tan, Li; Wu, Wanlei; Tang, Shi; Xiong, Zhou; Zheng, Shangen.
Afiliação
  • Liu W; Department of Transfusion, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei, China.
  • Liu L; Department of Transfusion, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei, China.
  • Kou G; Department of Transfusion, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei, China.
  • Zheng Y; Department of Transfusion, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei, China.
  • Ding Y; Department of Transfusion, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei, China.
  • Ni W; Department of Transfusion, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei, China.
  • Wang Q; Department of Disease Control and Prevention, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei, China.
  • Tan L; Department of Disease Control and Prevention, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei, China.
  • Wu W; Department of Transfusion, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei, China.
  • Tang S; Department of Transfusion, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei, China.
  • Xiong Z; Department of Transfusion, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei, China.
  • Zheng S; Department of Transfusion, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei, China sxkzsg@sina.com.
J Clin Microbiol ; 58(6)2020 05 26.
Article em En | MEDLINE | ID: mdl-32229605
At present, PCR-based nucleic acid detection cannot meet the demands for coronavirus infectious disease (COVID-19) diagnosis. Two hundred fourteen confirmed COVID-19 patients who were hospitalized in the General Hospital of Central Theater Command of the People's Liberation Army between 18 January and 26 February 2020 were recruited. Two enzyme-linked immunosorbent assay (ELISA) kits based on recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (rN) and spike protein (rS) were used for detecting IgM and IgG antibodies, and their diagnostic feasibility was evaluated. Among the 214 patients, 146 (68.2%) and 150 (70.1%) were successfully diagnosed with the rN-based IgM and IgG ELISAs, respectively; 165 (77.1%) and 159 (74.3%) were successfully diagnosed with the rS-based IgM and IgG ELISAs, respectively. The positive rates of the rN-based and rS-based ELISAs for antibody (IgM and/or IgG) detection were 80.4% and 82.2%, respectively. The sensitivity of the rS-based ELISA for IgM detection was significantly higher than that of the rN-based ELISA. We observed an increase in the positive rate for IgM and IgG with an increasing number of days post-disease onset (d.p.o.), but the positive rate of IgM dropped after 35 d.p.o. The positive rate of rN-based and rS-based IgM and IgG ELISAs was less than 60% during the early stage of the illness, 0 to 10 d.p.o., and that of IgM and IgG was obviously increased after 10 d.p.o. ELISA has a high sensitivity, especially for the detection of serum samples from patients after 10 d.p.o., so it could be an important supplementary method for COVID-19 diagnosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia Viral / Ensaio de Imunoadsorção Enzimática / Infecções por Coronavirus / Técnicas de Laboratório Clínico / Glicoproteína da Espícula de Coronavírus / Betacoronavirus Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Revista: J Clin Microbiol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia Viral / Ensaio de Imunoadsorção Enzimática / Infecções por Coronavirus / Técnicas de Laboratório Clínico / Glicoproteína da Espícula de Coronavírus / Betacoronavirus Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Revista: J Clin Microbiol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China