Protective activity of ellagic acid in counteract oxidative stress damage in zebrafish embryonic development.
Ecotoxicol Environ Saf
; 197: 110642, 2020 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-32311610
ABSTRACT
During its development, embryo is easily susceptible to reactive oxygen species (ROS). Evidence demonstrate protective role of the antioxidants, improving both cellular growth and embryonic development. Among these, ellagic acid (EA) is a natural antioxidant with anti-inflammatory and anti-carcinogen properties. The aim of this work was to assess in vitro the protective and anti-genotoxic role of EA during Danio rerio (zebrafish) embryonic development. For the study, zebrafish embryos were treated with H2O2 (15 µM, 30 µM and 45 µM) to simulate an oxidative damage, and with EA (2.5 mM, 5 mM and 10 mM) for 8, 20, 24, 48, 96 hpf (hours post fertilization). Vitality rate, alterations in the morphology and behavior of the larvae and the genomic stability were analyzed. The exposure to H2O2 caused genotoxicity for all exposure times. The incubation in 45 µM H2O2 and 30 µM H2O2 resulted in increased mortality rate of the larvae, as well as 10 mM EA. The co-exposure was performed using to 15 µM H2O2 and 2.5 mM and 5 mM EA and it demonstrated the EA capacity to protect the embryo DNA and development from the oxidative insult. Particularly, the co-exposure to 15 mM H2O2 and 5 mM EA showed an increase in the embryo survival rate and absence of alterations in morphology and behavior at 96 hpf. Interestingly, we observed a higher genomic stability at 8h and 20h co-exposure (15 mM H2O2 and 5 mM EA) time. The decline observed in ROS concentration for both exposure times confirmed the observation. In conclusion, EA protects the zebrafish embryonic development from DNA oxidative damage increasing the embryo survival rate and improving morphological parameters of the larvae.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dano ao DNA
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Peixe-Zebra
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Estresse Oxidativo
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Desenvolvimento Embrionário
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Ácido Elágico
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Embrião não Mamífero
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Antioxidantes
Limite:
Animals
Idioma:
En
Revista:
Ecotoxicol Environ Saf
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Itália