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The SEPT12 complex is required for the establishment of a functional sperm head-tail junction.
Shen, Yi-Ru; Wang, Han-Yu; Tsai, Yung-Chieh; Kuo, Yung-Che; Wu, Shang-Rung; Wang, Chia-Yih; Kuo, Pao-Lin.
Afiliação
  • Shen YR; Department of Obstetrics and Gynecology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Wang HY; Department of Obstetrics and Gynecology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Tsai YC; Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Kuo YC; Department of Obstetrics and Gynecology, Chi-Mei Medical Center, Tainan, Taiwan.
  • Wu SR; Department of Medicine, Taipei Medical University, Taipei, Taiwan.
  • Wang CY; Department of Sport Management, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.
  • Kuo PL; Department of Obstetrics and Gynecology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Mol Hum Reprod ; 26(6): 402-412, 2020 06 01.
Article em En | MEDLINE | ID: mdl-32392324
ABSTRACT
The connecting pieces of the sperm neck link the flagellum and the sperm head, and they are important for initiating flagellar beating. The connecting pieces are important building blocks for the sperm neck; however, the mechanism of connecting piece assembly is poorly understood. In the present study, we explored the role of septins in sperm motility and found that Sept12D197N knock-in (KI) mice produce acephalic and immotile spermatozoa. Electron microscopy analysis showed defective connecting pieces in sperm from KI mice, indicating that SEPT12 is required for the establishment of connecting pieces. We also found that SEPT12 formed a complex with SEPT1, SEPT2, SEPT10 and SEPT11 at the sperm neck and that the D197N mutation disrupted the complex, suggesting that the SEPT12 complex is involved in the assembly of connecting pieces. Additionally, we found that SEPT12 interacted and colocalized with γ-tubulin in elongating spermatids, implying that SEPT12 and pericentriolar materials jointly contribute to the formation of connecting pieces. Collectively, our findings suggest that SEPT12 is required for the formation of striated columns, and the capitulum and for maintaining the stability of the sperm head-tail junction.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatogênese / Espermatozoides / Septinas Limite: Animals Idioma: En Revista: Mol Hum Reprod Assunto da revista: BIOLOGIA MOLECULAR / MEDICINA REPRODUTIVA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Taiwan

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatogênese / Espermatozoides / Septinas Limite: Animals Idioma: En Revista: Mol Hum Reprod Assunto da revista: BIOLOGIA MOLECULAR / MEDICINA REPRODUTIVA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Taiwan