Loss of proton/calcium exchange 1 results in the activation of plant defense and accelerated senescence in Arabidopsis.
Plant Sci
; 296: 110472, 2020 Jul.
Article
em En
| MEDLINE
| ID: mdl-32540002
ABSTRACT
Cytosolic Ca2+ increases in response to many stimuli. CAX1 (H+/Ca2+ exchanger 1) maintains calcium homeostasis by transporting calcium from the cytosol to vacuoles. Here, we determined that the cax1 mutant exhibits enhanced resistance against both an avirulent biotrophic pathogen Pst-avrRpm1 (Pseudomonas syringae pv tomato DC3000 avrRpm1), and a necrotrophic pathogen, B. cinerea (Botrytis cinerea). The defense hormone SA (salicylic acid) and phytoalexin scopoletin, which fight against biotrophs and necrotrophs respectively, accumulated more in cax1 than wild-type. Moreover, the cax1 mutant exhibited early senescence after exogenous Ca2+ application. The accelerated senescence in the cax1 mutant was dependent on SID2 (salicylic acid induction deficient 2) but not on NPR1 (nonexpressor of pathogenesis-related genes1). Additionally, the introduction of CAX1 into the cax1 mutant resulted in phenotypes similar to that of wild-type in terms of Ca2+-conditioned senescence and Pst-avrRpm1 and B. cinerea infections. However, disruption of CAX3, the homolog of CAX1, did not produce an obvious phenotype. Moreover, exogenous Ca2+ application on plants resulted in increased resistance to both Pst-avrRpm1 and B. cinerea. Therefore, we conclude that the disruption of CAX1, but not CAX3, causes the activation of pathogen defense mechanisms, probably through the manipulation of calcium homeostasis or other signals.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cálcio
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Arabidopsis
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Antiporters
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Proteínas de Transporte de Cátions
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Proteínas de Arabidopsis
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Resistência à Doença
Idioma:
En
Revista:
Plant Sci
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China