Status of IL-4 and IL-10 driven markers in experimental models of Visceral Leishmaniasis.
Parasite Immunol
; 43(1): e12783, 2021 01.
Article
em En
| MEDLINE
| ID: mdl-32734677
ABSTRACT
AIM:
Leishmania donovani, the causative agent for visceral leishmaniasis (VL), modulates host monocytes/macrophages to ensure its survival. However, knowledge regarding the host-parasite interactions underpinning the disease remains limited. As disease progression is associated with polarization of monocytes/macrophages towards M2, which is regulated by cytokines IL-4/IL-13 and IL-10, this study evaluated the status of key IL-4- and IL-10 driven markers in experimental models of VL, as also evaluated their correlation, if any, with parasite load.METHODS:
In liver and splenic tissues from L donovani-infected hamsters and BALB/c mice, the parasite burden was determined along with mRNA expression of IL-4-driven markers, that is CD206, Arginase-I, CCL17, CCL22, PPAR-γ, STAT6, KLF4, FIZZ1 and YM1 along with IL-10-driven markers, CXCL13, IL-10, TGF-ß, VDR, CCR2 and CYP27A1.RESULTS:
The mRNA expression of IL-4- and IL-10-driven markers was enhanced in both models, but only in the hamster model, the splenic tissues demonstrated a positive correlation between all the IL-10-driven markers and parasite load.CONCLUSIONS:
Contrary to human VL, both models demonstrated an increased expression of IL-4- and IL-10-driven markers.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Interleucina-4
/
Interleucina-10
/
Leishmaniose Visceral
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Parasite Immunol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Índia