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Endogenous Retrovirus-Derived lncRNA BANCR Promotes Cardiomyocyte Migration in Humans and Non-human Primates.
Wilson, Kitchener D; Ameen, Mohamed; Guo, Hongchao; Abilez, Oscar J; Tian, Lei; Mumbach, Maxwell R; Diecke, Sebastian; Qin, Xulei; Liu, Yonggang; Yang, Huaxiao; Ma, Ning; Gaddam, Sadhana; Cunningham, Nathan J; Gu, Mingxia; Neofytou, Evgenios; Prado, Maricela; Hildebrandt, Thomas B; Karakikes, Ioannis; Chang, Howard Y; Wu, Joseph C.
Afiliação
  • Wilson KD; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA; Department of Pathology, Stanford University, Stanford, CA 94305, USA. Electronic address: kitch.wilson@gmail.com.
  • Ameen M; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA; Department of Cancer Biology, Stanford University, Stanford, CA 94305, USA.
  • Guo H; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
  • Abilez OJ; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
  • Tian L; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
  • Mumbach MR; Center for Personal Dynamic Regulomes and Program in Epithelial Biology, Stanford University, Stanford, CA 94305, USA.
  • Diecke S; Berlin Institute of Health, Max Delbrück Center, and DZHK (German Center for Cardiovascular Research), Berlin, Germany.
  • Qin X; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
  • Liu Y; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
  • Yang H; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
  • Ma N; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
  • Gaddam S; Center for Personal Dynamic Regulomes and Program in Epithelial Biology, Stanford University, Stanford, CA 94305, USA.
  • Cunningham NJ; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
  • Gu M; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
  • Neofytou E; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
  • Prado M; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
  • Hildebrandt TB; Wildlife Reproduction Medicine, Freie University and Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany.
  • Karakikes I; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA; Department of Cardiothoracic Surgery, Stanford University, Stanford, CA 94305, USA.
  • Chang HY; Center for Personal Dynamic Regulomes and Program in Epithelial Biology, Stanford University, Stanford, CA 94305, USA.
  • Wu JC; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA; Departments of Medicine and Radiology, Stanford University, Stanford, CA 94305, USA. Electronic address: joewu@stanford.edu.
Dev Cell ; 54(6): 694-709.e9, 2020 09 28.
Article em En | MEDLINE | ID: mdl-32763147
ABSTRACT
Transposable elements (TEs) comprise nearly half of the human genome and are often transcribed or exhibit cis-regulatory properties with unknown function in specific processes such as heart development. In the case of endogenous retroviruses (ERVs), a TE subclass, experimental interrogation is constrained as many are primate-specific or human-specific. Here, we use primate pluripotent stem-cell-derived cardiomyocytes that mimic fetal cardiomyocytes in vitro to discover hundreds of ERV transcripts from the primate-specific MER41 family, some of which are regulated by the cardiogenic transcription factor TBX5. The most significant of these are located within BANCR, a long non-coding RNA (lncRNA) exclusively expressed in primate fetal cardiomyocytes. Functional studies reveal that BANCR promotes cardiomyocyte migration in vitro and ventricular enlargement in vivo. We conclude that recently evolved TE loci such as BANCR may represent potent de novo developmental regulatory elements that can be interrogated with species-matching pluripotent stem cell models.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Retrovirus Endógenos / Miócitos Cardíacos / RNA Longo não Codificante Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Dev Cell Assunto da revista: EMBRIOLOGIA Ano de publicação: 2020 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Retrovirus Endógenos / Miócitos Cardíacos / RNA Longo não Codificante Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Dev Cell Assunto da revista: EMBRIOLOGIA Ano de publicação: 2020 Tipo de documento: Article