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Actin chromobody imaging reveals sub-organellar actin dynamics.
Schiavon, Cara R; Zhang, Tong; Zhao, Bing; Moore, Andrew S; Wales, Pauline; Andrade, Leonardo R; Wu, Melissa; Sung, Tsung-Chang; Dayn, Yelena; Feng, Jasmine W; Quintero, Omar A; Shadel, Gerald S; Grosse, Robert; Manor, Uri.
Afiliação
  • Schiavon CR; Waitt Advanced Biophotonics Center, Salk Institute for Biological Studies, La Jolla, CA, USA.
  • Zhang T; Molecular and Cellular Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.
  • Zhao B; Waitt Advanced Biophotonics Center, Salk Institute for Biological Studies, La Jolla, CA, USA.
  • Moore AS; Institute of Experimental and Clinical Pharmacology and Toxicology, University of Freiburg, Freiburg im Breisgau, Germany.
  • Wales P; Janelia Research Campus, Howard Hughes Medical Institute, Chevy Chase, MD, USA.
  • Andrade LR; Waitt Advanced Biophotonics Center, Salk Institute for Biological Studies, La Jolla, CA, USA.
  • Wu M; Molecular and Cellular Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.
  • Sung TC; Waitt Advanced Biophotonics Center, Salk Institute for Biological Studies, La Jolla, CA, USA.
  • Dayn Y; Waitt Advanced Biophotonics Center, Salk Institute for Biological Studies, La Jolla, CA, USA.
  • Feng JW; Transgenic Core, Salk Institute for Biological Studies, La Jolla, CA, USA.
  • Quintero OA; Transgenic Core, Salk Institute for Biological Studies, La Jolla, CA, USA.
  • Shadel GS; Department of Biology, University of Richmond, Richmond, VA, USA.
  • Grosse R; Department of Biology, University of Richmond, Richmond, VA, USA.
  • Manor U; Molecular and Cellular Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.
Nat Methods ; 17(9): 917-921, 2020 09.
Article em En | MEDLINE | ID: mdl-32778832
The actin cytoskeleton plays multiple critical roles in cells, from cell migration to organelle dynamics. The small and transient actin structures regulating organelle dynamics are challenging to detect with fluorescence microscopy, making it difficult to determine whether actin filaments are directly associated with specific membranes. To address these limitations, we developed fluorescent-protein-tagged actin nanobodies, termed 'actin chromobodies' (ACs), targeted to organelle membranes to enable high-resolution imaging of sub-organellar actin dynamics.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citoesqueleto de Actina / Imagem Óptica Limite: Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
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PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citoesqueleto de Actina / Imagem Óptica Limite: Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos