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Time-resolved cryo-EM using Spotiton.
Dandey, Venkata P; Budell, William C; Wei, Hui; Bobe, Daija; Maruthi, Kashyap; Kopylov, Mykhailo; Eng, Edward T; Kahn, Peter A; Hinshaw, Jenny E; Kundu, Nidhi; Nimigean, Crina M; Fan, Chen; Sukomon, Nattakan; Darst, Seth A; Saecker, Ruth M; Chen, James; Malone, Brandon; Potter, Clinton S; Carragher, Bridget.
Afiliação
  • Dandey VP; The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
  • Budell WC; The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
  • Wei H; The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
  • Bobe D; The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
  • Maruthi K; The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
  • Kopylov M; The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
  • Eng ET; The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
  • Kahn PA; Engineering Arts LLC, Tempe, AZ, USA.
  • Hinshaw JE; Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
  • Kundu N; Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
  • Nimigean CM; Department of Anesthesiology, Weill Cornell Medical College, New York, NY, USA.
  • Fan C; Department of Anesthesiology, Weill Cornell Medical College, New York, NY, USA.
  • Sukomon N; Department of Anesthesiology, Weill Cornell Medical College, New York, NY, USA.
  • Darst SA; Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY, USA.
  • Saecker RM; Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY, USA.
  • Chen J; Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY, USA.
  • Malone B; Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY, USA.
  • Potter CS; The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
  • Carragher B; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
Nat Methods ; 17(9): 897-900, 2020 09.
Article em En | MEDLINE | ID: mdl-32778833
ABSTRACT
We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study short-lived molecular states. Using piezoelectric dispensing, two independent streams of ~50-pl droplets of sample are deposited within 10 ms of each other onto the surface of a nanowire EM grid, and the mixing reaction stops when the grid is vitrified in liquid ethane ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microscopia Crioeletrônica Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microscopia Crioeletrônica Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos