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Direct and Indirect Effects of Increased CO2 Partial Pressure on the Bioenergetics of Syntrophic Propionate and Butyrate Conversion.
Ceron-Chafla, Pamela; Kleerebezem, Robbert; Rabaey, Korneel; van Lier, Jules B; Lindeboom, Ralph E F.
Afiliação
  • Ceron-Chafla P; Sanitary Engineering Section, Department of Water Management, Delft University of Technology, Stevinweg 1, 2628 CN Delft, The Netherlands.
  • Kleerebezem R; Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, Netherlands.
  • Rabaey K; Center for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
  • van Lier JB; Center for Advanced Process Technology for Urban Resource Recovery (CAPTURE), Coupure Links 653, B-9000 Ghent, Belgium.
  • Lindeboom REF; Sanitary Engineering Section, Department of Water Management, Delft University of Technology, Stevinweg 1, 2628 CN Delft, The Netherlands.
Environ Sci Technol ; 54(19): 12583-12592, 2020 10 06.
Article em En | MEDLINE | ID: mdl-32845128
ABSTRACT
Simultaneous digestion and in situ biogas upgrading in high-pressure bioreactors will result in elevated CO2 partial pressure (pCO2). With the concomitant increase in dissolved CO2, microbial conversion processes may be affected beyond the impact of increased acidity. Elevated pCO2 was reported to affect the kinetics and thermodynamics of biochemical conversions because CO2 is an intermediate and end-product of the digestion process and modifies the carbonate equilibrium. Our results showed that increasing pCO2 from 0.3 to 8 bar in lab-scale batch reactors decreased the maximum substrate utilization rate (rsmax) for both syntrophic propionate and butyrate oxidation. These kinetic limitations are linked to an increased overall Gibbs free energy change (ΔGOverall) and a potential biochemical energy redistribution among syntrophic partners, which showed interdependence with hydrogen partial pressure (pH2). The bioenergetics analysis identified a moderate, direct impact of elevated pCO2 on propionate oxidation and a pH-mediated effect on butyrate oxidation. These constraints, combined with physiological limitations on growth exerted by increased acidity and inhibition due to higher concentrations of undissociated volatile fatty acids, help to explain the observed phenomena. Overall, this investigation sheds light on the role of elevated pCO2 in delicate biochemical syntrophic conversions by connecting kinetic, bioenergetic, and physiological effects.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Propionatos / Butiratos Idioma: En Revista: Environ Sci Technol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Propionatos / Butiratos Idioma: En Revista: Environ Sci Technol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Holanda