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Association between low-to-moderate fluoride exposure and bone mineral density in Chinese adults: Non-negligible role of RUNX2 promoter methylation.
Gao, Minghui; Sun, Long; Xu, Kaihong; Zhang, Luoming; Zhang, Yanli; He, Tongkun; Sun, Renjie; Huang, Hui; Zhu, Jingyuan; Zhang, Yawei; Zhou, Guoyu; Ba, Yue.
Afiliação
  • Gao M; Department of Occupational and Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; Environment and Health Innovation Team, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China. Electronic address: mavis_gmh@163.com.
  • Sun L; Department of Endemic Disease, Kaifeng Center for Disease Control and Prevention, Kaifeng, Henan, 475004, PR China. Electronic address: kfcdcbgs@126.com.
  • Xu K; Department of Occupational and Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China. Electronic address: kaihhongong@163.com.
  • Zhang L; Department of Endemic Disease, Kaifeng Center for Disease Control and Prevention, Kaifeng, Henan, 475004, PR China. Electronic address: 18903789068@163.com.
  • Zhang Y; Department of Occupational and Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China. Electronic address: zhangylzzu@163.com.
  • He T; Department of Occupational and Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China. Electronic address: 13939042293@163.com.
  • Sun R; Department of Occupational and Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China. Electronic address: allensrj@qq.com.
  • Huang H; Department of Occupational and Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; Environment and Health Innovation Team, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China. Electronic address: huihuang@zzu.edu.cn.
  • Zhu J; Department of Occupational and Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China. Electronic address: yuanzhu@zzu.edu.cn.
  • Zhang Y; Department of Environmental Health Sciences, School of Public Health, Yale University, New Haven, CT, 06520, USA. Electronic address: yawei.zhang@yale.edu.
  • Zhou G; Department of Occupational and Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; Environment and Health Innovation Team, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; Yellow River Institute for Ecological Pro
  • Ba Y; Department of Occupational and Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; Environment and Health Innovation Team, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; Yellow River Institute for Ecological Pro
Ecotoxicol Environ Saf ; 203: 111031, 2020 Oct 15.
Article em En | MEDLINE | ID: mdl-32888610
Bone mineral density (BMD) changes were reported to be associated with excessive fluoride exposure and abnormal expression of RUNX2. However, whether the alteration of methylation status, a most commonly used marker for the alteration of gene expression in epidemiological investigation, of RUNX2 is associated with low-to-moderate fluoride exposure and BMD changes has not been reported. Our study aims to explore the role of RUNX2 promoter methylation in BMD changes induced by low-to-moderate fluoride exposure. A total of 1124 adults (413 men and 711 women) were recruited from Kaifeng City in 2017. We measured BMD using ultrasound bone densitometer. Concentrations of urinary fluoride (UF) were measured using ion-selective electrode, and the participants were grouped into control group (CG) and excessive fluoride group (EFG) according to the concentration of UF. We extracted DNA from fasting peripheral blood samples and then detected the promoter methylation levels of RUNX2 using quantitative methylation-specific PCR. Relationships between UF concentration, RUNX2 promoter methylation and BMD changes were analyzed using generalized linear model and logistic regression. Results showed in EFG (UF concentration > 1.6 mg/L), BMD was negatively correlated with UF concentration (ß: -0.14; 95%CI: -0.26, -0.01) and RUNX2 promoter methylation (ß: -0.13; 95%CI: -0.22, -0.03) in women. The methylation rate of RUNX2 promoter increased by 2.16% for each 1 mg/L increment in UF concentration of women in EFG (95%CI: 0.37, 3.96). No any significant associations between UF concentration, RUNX2 promoter methylation, and BMD were observed in the individuals in CG. Mediation analysis showed that RUNX2 promoter methylation mediated 18.2% (95% CI: 4.2%, 53.2%) of the association between UF concentration and BMD of women in EFG. In conclusion, excessive fluoride exposure (>1.6 mg/L) is associated with changes of BMD in women, and this association is mediated by RUNX2 promoter methylation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Densidade Óssea / Exposição Ambiental / Subunidade alfa 1 de Fator de Ligação ao Core / Fluoretos Tipo de estudo: Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged País/Região como assunto: Asia Idioma: En Revista: Ecotoxicol Environ Saf Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Densidade Óssea / Exposição Ambiental / Subunidade alfa 1 de Fator de Ligação ao Core / Fluoretos Tipo de estudo: Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged País/Região como assunto: Asia Idioma: En Revista: Ecotoxicol Environ Saf Ano de publicação: 2020 Tipo de documento: Article