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Prokaryotic viperins produce diverse antiviral molecules.
Bernheim, Aude; Millman, Adi; Ofir, Gal; Meitav, Gilad; Avraham, Carmel; Shomar, Helena; Rosenberg, Masha M; Tal, Nir; Melamed, Sarah; Amitai, Gil; Sorek, Rotem.
Afiliação
  • Bernheim A; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
  • Millman A; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
  • Ofir G; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
  • Meitav G; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
  • Avraham C; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
  • Shomar H; Pantheon Biosciences, Yavne, Israel.
  • Rosenberg MM; Pantheon Biosciences, Yavne, Israel.
  • Tal N; Pantheon Biosciences, Yavne, Israel.
  • Melamed S; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
  • Amitai G; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
  • Sorek R; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel. rotem.sorek@weizmann.ac.il.
Nature ; 589(7840): 120-124, 2021 01.
Article em En | MEDLINE | ID: mdl-32937646
Viperin is an interferon-induced cellular protein that is conserved in animals1. It has previously been shown to inhibit the replication of multiple viruses by producing the ribonucleotide 3'-deoxy-3',4'-didehydro (ddh)-cytidine triphosphate (ddhCTP), which acts as a chain terminator for viral RNA polymerase2. Here we show that eukaryotic viperin originated from a clade of bacterial and archaeal proteins that protect against phage infection. Prokaryotic viperins produce a set of modified ribonucleotides that include ddhCTP, ddh-guanosine triphosphate (ddhGTP) and ddh-uridine triphosphate (ddhUTP). We further show that prokaryotic viperins protect against T7 phage infection by inhibiting viral polymerase-dependent transcription, suggesting that it has an antiviral mechanism of action similar to that of animal viperin. Our results reveal a class of potential natural antiviral compounds produced by bacterial immune systems.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antivirais / Células Procarióticas / Proteínas de Bactérias / Proteínas / Bacteriófago T7 / Evolução Molecular / Proteínas Arqueais Limite: Humans Idioma: En Revista: Nature Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Israel

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antivirais / Células Procarióticas / Proteínas de Bactérias / Proteínas / Bacteriófago T7 / Evolução Molecular / Proteínas Arqueais Limite: Humans Idioma: En Revista: Nature Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Israel