Development of a specific immunoassay to selectively measure active tryptase in airway samples.
Bioanalysis
; 12(19): 1377-1388, 2020 Oct.
Article
em En
| MEDLINE
| ID: mdl-32975431
ABSTRACT
Aim:
Tryptase is a tetrameric trypsin-like serine protease contained within the secretory granules of mast cells and is an important mediator of allergic inflammatory responses in respiratory diseases. Detection of active tryptase in the airway may provide important information about asthma and other respiratory diseases. Materials &Methods:
An activity based probe has been incorported within an immunoassay to allow for measurement of active tryptase in human tissues.Results:
A specific Simoa immunoassay to measure active tryptase in nasosorption samples was developed and qualified using an activity-based probe label and a specific antitryptase capture antibody.Conclusion:
The assay was capable of measuring active tryptase in human samples, which will enable evaluation of the role of tryptase proteolytic activity in human disease.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testes Imunológicos
/
Imunoensaio
/
Triptases
/
Mastócitos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos