Your browser doesn't support javascript.
loading
The atypical chemokine receptor 3 interacts with Connexin 43 inhibiting astrocytic gap junctional intercellular communication.
Fumagalli, Amos; Heuninck, Joyce; Pizzoccaro, Anne; Moutin, Enora; Koenen, Joyce; Séveno, Martial; Durroux, Thierry; Junier, Marie-Pierre; Schlecht-Louf, Géraldine; Bachelerie, Francoise; Schütz, Dagmar; Stumm, Ralf; Smit, Martine J; Guérineau, Nathalie C; Chaumont-Dubel, Séverine; Marin, Philippe.
Afiliação
  • Fumagalli A; Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Heuninck J; Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Pizzoccaro A; Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Moutin E; Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Koenen J; Université Paris-Saclay, Inserm, Inflammation, Microbiome and Immunosurveillance, 92140, Clamart, France.
  • Séveno M; Amsterdam Institute for Molecules Medicines and Systems, Division of Medicinal Chemistry, Faculty of Sciences, VU University Amsterdam, 1081, HV, Amsterdam, The Netherlands.
  • Durroux T; Biocampus Montpellier, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Junier MP; Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Schlecht-Louf G; CNRS UMR8246, Inserm U1130, Neuroscience Paris Seine-IBPS, Sorbonne Universités, Paris, France.
  • Bachelerie F; Université Paris-Saclay, Inserm, Inflammation, Microbiome and Immunosurveillance, 92140, Clamart, France.
  • Schütz D; Université Paris-Saclay, Inserm, Inflammation, Microbiome and Immunosurveillance, 92140, Clamart, France.
  • Stumm R; Institute of Pharmacology and Toxicology, Jena University Hospital, 07747, Jena, Germany.
  • Smit MJ; Institute of Pharmacology and Toxicology, Jena University Hospital, 07747, Jena, Germany.
  • Guérineau NC; Amsterdam Institute for Molecules Medicines and Systems, Division of Medicinal Chemistry, Faculty of Sciences, VU University Amsterdam, 1081, HV, Amsterdam, The Netherlands.
  • Chaumont-Dubel S; Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Marin P; Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, Montpellier, France.
Nat Commun ; 11(1): 4855, 2020 09 25.
Article em En | MEDLINE | ID: mdl-32978390
ABSTRACT
The atypical chemokine receptor 3 (ACKR3) plays a pivotal role in directing the migration of various cellular populations and its over-expression in tumors promotes cell proliferation and invasiveness. The intracellular signaling pathways transducing ACKR3-dependent effects remain poorly characterized, an issue we addressed by identifying the interactome of ACKR3. Here, we report that recombinant ACKR3 expressed in HEK293T cells recruits the gap junction protein Connexin 43 (Cx43). Cx43 and ACKR3 are co-expressed in mouse brain astrocytes and human glioblastoma cells and form a complex in embryonic mouse brain. Functional in vitro studies show enhanced ACKR3 interaction with Cx43 upon ACKR3 agonist stimulation. Furthermore, ACKR3 activation promotes ß-arrestin2- and dynamin-dependent Cx43 internalization to inhibit gap junctional intercellular communication in primary astrocytes. These results demonstrate a functional link between ACKR3 and gap junctions that might be of pathophysiological relevance.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Comunicação Celular / Astrócitos / Junções Comunicantes / Conexina 43 / Receptores CXCR Limite: Animals / Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: França

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Comunicação Celular / Astrócitos / Junções Comunicantes / Conexina 43 / Receptores CXCR Limite: Animals / Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: França