Genetic Features Leading to Reduced Susceptibility to Aztreonam-Avibactam among Metallo-ß-Lactamase-Producing Escherichia coli Isolates.
Antimicrob Agents Chemother
; 64(12)2020 11 17.
Article
em En
| MEDLINE
| ID: mdl-32988825
ABSTRACT
Metallo-ß-lactamase (MBL)-producing Escherichia coli isolates resistant to the newly developed ß-lactam/ß-lactamase inhibitor drug combination aztreonam-avibactam (ATM-AVI) have been reported. Here, we analyzed a series of 118 clinical MBL-producing E. coli isolates of various geographical origins for susceptibility to ATM-AVI. The nature of the PBP3 protein sequence and the occurrence of blaCMY genes for susceptibility to ATM-AVI were investigated. We showed here that elevated MICs of ATM-AVI among MBL-producing E. coli isolates resulted from a combination of different features, including modification of PBP3 protein sequence through specific amino acid insertions and production of CMY-type enzymes, particularly, CMY-42. We showed here that those insertions identified in the PBP3 sequence are not considered the unique basis of resistance to ATM-AVI, but they significantly contribute to it.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Beta-Lactamases
/
Aztreonam
Idioma:
En
Revista:
Antimicrob Agents Chemother
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
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