Mammalian Atg8-family proteins are upstream regulators of the lysosomalsystem by controlling MTOR and TFEB.
Autophagy
; 16(12): 2305-2306, 2020 12.
Article
em En
| MEDLINE
| ID: mdl-33070669
ABSTRACT
Macroautophagy/autophagy delivers cytoplasmic cargo to lysosomes for degradation. In yeast, the single Atg8 protein plays a role in the formation of autophagosomes whereas in mammalian cells there are five to seven paralogs, referred to as mammalian Atg8s (mAtg8s GABARAP, GABARAPL1, GABARAPL2, LC3A, LC3B, LC3B2 and LC3C) with incompletely defined functions. Here we show that a subset of mAtg8s directly control lysosomal biogenesis. This occurs at the level of TFEB, the principal regulator of the lysosomal transcriptional program. mAtg8s promote TFEB's nuclear translocation in response to stimuli such as starvation. GABARAP interacts directly with TFEB, whereas RNA-Seq analyses reveal that knockout of six genes encoding mAtg8s, or a triple knockout of the genes encoding all GABARAPs, diminishes the TFEB transcriptional program. We furthermore show that GABARAPs in cooperation with other proteins, IRGM, a factor implicated in tuberculosis and Crohn disease, and STX17, are required during starvation for optimal inhibition of MTOR, an upstream kinase of TFEB, and activation of the PPP3/calcineurin phosphatase that dephosphorylates TFEB, thus promoting its nuclear translocation. In conclusion, mAtg8s, IRGM and STX17 control lysosomal biogenesis by their combined or individual effects on MTOR, TFEB, and PPP3/calcineurin, independently of their roles in the formation of autophagosomal membranes. Abbreviations AMPK AMP-activated protein kinase; IRGM immunity related GTPase M; mAtg8s mammalian Atg8 proteins; MTOR mechanistic target of rapamycin kinase; PPP3CB protein phosphatase 3 catalytic subunit beta; RRAGA Ras related GTP binding A.; STX17 syntaxin 17; ULK1 unc-51 like autophagy activating kinase 1.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Autophagy
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos