Highly Specific Mouse Anti-Joining Chain of Human Immunoglobulin A.
Monoclon Antib Immunodiagn Immunother
; 39(6): 228-232, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-33121367
ABSTRACT
Immunoglobulin A (IgA) antibodies are critical to mucosal protection, specifically dimeric IgA (dIgA) and secretory IgA (sIgA), which rely on the J chain to polymerize. There is an absence of monoclonal antibodies that can specifically bind to polymeric IgA without the need to denature the molecule. We generated a panel of highly specific mouse anti-J chain antibodies that react with both intact and denatured nonhuman primate dIgA and human dIgA and sIgA of both the IgA1 and IgA2 subclass. We expanded use of this antibody for quantification of dIgA and sIgA using biolayer interferometry or enzyme-linked immunosorbent assay and use for affinity chromatography. This is a significant improvement over available anti-IgA antibodies in the field, which will allow for expanded use in clinical testing.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina A
/
Imunoglobulina A Secretora
/
Anticorpos Monoclonais
/
Especificidade de Anticorpos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Monoclon Antib Immunodiagn Immunother
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos