A2AR Antagonists Upregulate Expression of GS and GLAST in Rat Hypoxia Model.
Biomed Res Int
; 2020: 2054293, 2020.
Article
em En
| MEDLINE
| ID: mdl-33195689
ABSTRACT
BACKGROUND:
The aim of this study was to research the effects of glutamine synthetase (GS) and glutamate aspartate transporter (GLAST) in rat Müller cells and the effects of an adenosine A2AR antagonist (SCH 442416) on GS and GLAST in hypoxia both in vivo and in vitro.METHODS:
This study used RT-PCR and Western blotting to quantify the expressions of GS and GLAST under different hypoxic conditions as well as the expressions of GS and GLAST at different drug concentrations. A cell viability assay was used to assess drug toxicity.RESULTS:
mRNA and protein expression of GS and GLAST in hypoxia Group 24 h was significantly increased. mRNA and protein expressions of GS and GLAST both increased in Group 1 µM SCH 442416 compared with other groups. One micromolar SCH 442416 could upregulate GS and GLAST's activity in hypoxia both in vivo and in vitro.CONCLUSIONS:
Hypoxia activates GS and GLAST in rat retinal Müller cells in a short time in vitro. (2) A2AR antagonists upregulate the activity of GS and GLAST in hypoxia both in vivo and in vitro.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação para Cima
/
Sistema X-AG de Transporte de Aminoácidos
/
Antagonistas do Receptor A2 de Adenosina
/
Glutamato-Amônia Ligase
/
Hipóxia
Limite:
Animals
Idioma:
En
Revista:
Biomed Res Int
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China