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Analytical methodologies for the detection of SARS-CoV-2 in wastewater: Protocols and future perspectives.
Alygizakis, Nikiforos; Markou, Athina N; Rousis, Nikolaos I; Galani, Aikaterini; Avgeris, Margaritis; Adamopoulos, Panagiotis G; Scorilas, Andreas; Lianidou, Evi S; Paraskevis, Dimitrios; Tsiodras, Sotirios; Tsakris, Athanassios; Dimopoulos, Meletios-Athanasios; Thomaidis, Nikolaos S.
Afiliação
  • Alygizakis N; Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Greece.
  • Markou AN; Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Greece.
  • Rousis NI; Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Greece.
  • Galani A; Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Greece.
  • Avgeris M; Laboratory of Clinical Biochemistry and Molecular Diagnostics, Second Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
  • Adamopoulos PG; Laboratory of Biochemistry and Molecular Biology, Department of Biology, National and Kapodistrian University of Athens, Greece.
  • Scorilas A; Laboratory of Biochemistry and Molecular Biology, Department of Biology, National and Kapodistrian University of Athens, Greece.
  • Lianidou ES; Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Greece.
  • Paraskevis D; Department of Hygiene Epidemiology and Medical Statistics, School of Medicine, National and Kapodistrian University of Athens, Greece.
  • Tsiodras S; Fourth Department of Propedeutic Internal Medicine, School of Medicine, University General Hospital Attikon, National and Kapodistrian University of Athens, Greece.
  • Tsakris A; Department of Microbiology, School of Medicine, National and Kapodistrian University of Athens, Greece.
  • Dimopoulos MA; Department of Clinical Therapeutics, Medical School, National and Kapodistrian University of Athens, Greece.
  • Thomaidis NS; Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Greece.
Trends Analyt Chem ; 134: 116125, 2021 Jan.
Article em En | MEDLINE | ID: mdl-33235400
In March 2020 the World Health Organization announced a pandemic outbreak. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative pathogen for the coronavirus disease-19 (COVID-19) pandemic. The authorities worldwide use clinical science to identify infected people, but this approach is not able to track all symptomatic and asymptomatic cases due to limited sampling capacity of the testing laboratories. This drawback is eliminated by the Wastewater-Based Epidemiology (WBE) approach. In this review, we summarized the peer-reviewed published literature (available as of September 28, 2020), in the field of WBE. The commonly used steps (sampling, storage, concentration, isolation, detection) of the analytical protocols were identified. The potential limitations of each stage of the protocols and good practices were discussed. Finally, new methods for the efficient detection of SARS-CoV-2 were proposed.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Guideline / Systematic_reviews Idioma: En Revista: Trends Analyt Chem Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Grécia
Texto completo
Imprimir
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PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Guideline / Systematic_reviews Idioma: En Revista: Trends Analyt Chem Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Grécia