CRISPR-Based Approaches for Efficient and Accurate Detection of SARS-CoV-2.
Lab Med
; 52(2): 116-121, 2021 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-33316059
ABSTRACT
An outbreak of COVID-19, caused by infection with SARS-CoV-2 in Wuhan, China in December 2019, spread throughout the country and around the world, quickly. The primary detection technique for SARS-CoV-2, the reverse-transcription polymerase chain reaction (RT-PCR)-based approach, requires expensive reagents and equipment and skilled personnel. In addition, for SARS-CoV-2 detection, specimens are usually shipped to a designated laboratory for testing, which may extend the diagnosis and treatment time of patients with COVID-19. The latest research shows that clustered regularly interspaced short palindromic repeats (CRISPR)-based approaches can quickly provide visual, rapid, ultrasensitive, and specific detection of SARS-CoV-2 at isothermal conditions. Therefore, CRISPR-based approaches are expected to be developed as attractive alternatives to conventional RT-PCR methods for the efficient and accurate detection of SARS-CoV-2. Recent advances in the field of CRISPR-based biosensing technologies for SARS-CoV-2 detection and insights into their potential use in many applications are reviewed in this article.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
/
Teste de Ácido Nucleico para COVID-19
/
SARS-CoV-2
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Lab Med
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China