TRPA1 deficiency alleviates inflammation of atopic dermatitis by reducing macrophage infiltration.
Life Sci
; 266: 118906, 2021 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-33338502
ABSTRACT
AIMS:
The aim of this study was to investigate the role of TRPA1 in the pathogenesis of AD. MAINMETHODS:
The experimental atopic dermatitis (AD)-like skin lesions were established using 2,4-dinitrochlorobenzene (DNCB). Mice were divided into three groups TRPA1-/- and WT groups were treated with DNCB dissolved in a 31 mixture of acetone and olive oil; the negative control group was treated with 31 mixture of acetone and olive oil without DNCB. The treatment lasted for 21 days, after which the animals were sacrificed and their blood, ears and dorsal skin tissue samples were collected for analysis. KEYFINDINGS:
Lower dermatitis score, ear thickness, pruritus score, and epidermal hyperplasia were observed in mice in TRPA1-/- mice compared to the WT group. Besides, lower dermal mast cell infiltration, proinflammatory cytokines, Th2 cytokines and the infiltration of macrophages were observed in the TRPA1-/- mice compared to the WT group. Furthermore, we demonstrated that TRPA1 antagonist HC-030031 could alleviate AD-like symptoms and reduce the degree of epidermal hyperplasia in mice.SIGNIFICANCE:
TRPA1 has a crucial role during the AD pathogenesis in mice, thus may be used as a potential new target for treating patients with chronic skin inflammatory disease.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Prurido
/
Dermatite Atópica
/
Canal de Cátion TRPA1
/
Inflamação
/
Macrófagos
/
Mastócitos
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Life Sci
Ano de publicação:
2021
Tipo de documento:
Article