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The impact of technical failures on recombinant production of soluble proteins in Escherichia coli: a case study on process and protein robustness.
Pekarsky, Alexander; Reininger, Melanie; Spadiut, Oliver.
Afiliação
  • Pekarsky A; Institute of Chemical, Environmental and Bioscience Engineering, Research Area Biochemical Engineering, Technische Universität Wien, TU Wien, Gumpendorferstrasse 1a, 1060, Vienna, Austria.
  • Reininger M; Institute of Chemical, Environmental and Bioscience Engineering, Research Area Biochemical Engineering, Technische Universität Wien, TU Wien, Gumpendorferstrasse 1a, 1060, Vienna, Austria.
  • Spadiut O; Institute of Chemical, Environmental and Bioscience Engineering, Research Area Biochemical Engineering, Technische Universität Wien, TU Wien, Gumpendorferstrasse 1a, 1060, Vienna, Austria. oliver.spadiut@tuwien.ac.at.
Bioprocess Biosyst Eng ; 44(6): 1049-1061, 2021 Jun.
Article em En | MEDLINE | ID: mdl-33491129
ABSTRACT
Technical failures lead to deviations in process parameters that can exceed studied process boundaries. The impact on cell and target protein is often unknown. However, investigations on common technical failures might yield interesting insights into process and protein robustness. Recently, we published a study on the impact of technical failures on an inclusion body process that showed high robustness due to the inherent stability of IBs. In this follow-up study, we investigated the influence of technical failures during production of two soluble, cytosolic proteins in E. coli BL21(DE3). Cell physiology, productivity and protein quality were analyzed, after technical failures in aeration, substrate supply, temperature and pH control had been triggered. In most cases, cell physiology and productivity recovered during a subsequent regeneration phase. However, our results highlight that some technical failures lead to persistent deviations and affect the quality of purified protein.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Expressão Gênica / Escherichia coli Tipo de estudo: Observational_studies / Prognostic_studies Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Áustria

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Expressão Gênica / Escherichia coli Tipo de estudo: Observational_studies / Prognostic_studies Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Áustria