Effects of renalase deficiency on liver fibrosis markers in a nonalcoholic steatohepatitis mouse model.
Mol Med Rep
; 23(3)2021 03.
Article
em En
| MEDLINE
| ID: mdl-33495844
ABSTRACT
Progression of nonalcoholic steatohepatitis (NASH) is attributed to several factors, including inflammation and oxidative stress. In recent years, renalase has been reported to suppress oxidative stress, apoptosis and inflammation. A number of studies have suggested that renalase may be associated with protecting the liver from injury. The present study aimed to clarify the effects of renalase knockout (KO) in mice with NASH that were induced with a cholinedeficient highfat diet (CDAHFD) supplemented with 0.1% methionine. Wild type (WT) and KO mice (6weekold) were fed a normal diet (ND) or CDAHFD for 6 weeks, followed by analysis of the blood liver function markers and liver tissues. CDAHFD intake was revealed to increase blood hepatic function markers, lipid accumulation and oxidative stress compared with ND, but no significant differences were observed between the WT and KO mice. However, in the KOCDAHFD group, the Adgre1 and Tgfb1 mRNA levels were significantly higher, and αSMA expression was significantly lower compared with the WTCDAHFD group. Furthermore, the Gclc mRNA and phosphorylated protein kinase B (Akt) levels were significantly lower in the KOND group compared with the WTND group. The results of the current study indicated that as NASH progressed in the absence of renalase, oxidative stress, macrophage infiltration and TGFß expression were enhanced, while αSMA expression in NASH may be partly suppressed due to the decreased phosphorylation of Akt level.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
/
Regulação da Expressão Gênica
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Hepatopatia Gordurosa não Alcoólica
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Cirrose Hepática Experimental
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Monoaminoxidase
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Japão