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Icaritin and icariin reduce p-Tau levels in a cell model of Alzheimer's disease by downregulating glycogen synthase kinase 3ß.
Li, Ying; Dai, Shirui; Huang, Nanqu; Wu, Jie; Yu, Changyin; Luo, Yong.
Afiliação
  • Li Y; Department of Neurology, Third Affiliated Hospital of Zunyi Medical University/First People's Hospital of Zunyi, Zunyi, Guizhou, People's Republic of China.
  • Dai S; Department of Neurology, First People's Hospital of Guiyang, Guiyang, Guizhou, People's Republic of China.
  • Huang N; XiangYa School of Medicine, Central South University, Changsha, Hunan, People's Republic of China.
  • Wu J; Department of Neurology, Third Affiliated Hospital of Zunyi Medical University/First People's Hospital of Zunyi, Zunyi, Guizhou, People's Republic of China.
  • Yu C; Central Laboratory, Third Affiliated Hospital of Zunyi Medical University/First People's Hospital of Zunyi, Zunyi, Guizhou, People's Republic of China.
  • Luo Y; Department of Neurology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, People's Republic of China.
Biotechnol Appl Biochem ; 69(1): 355-363, 2022 Feb.
Article em En | MEDLINE | ID: mdl-33502043
ABSTRACT
This study aimed to explore the neuroprotective effect of icariin/icaritin (ICA/ICT) and the role of ICA/ICT in the treatment of Alzheimer's disease (AD). ICA and ICT were used to treat okadaic acid (OA)-induced Tau hyperphosphorylation in SH-SY5Y cells. We detected the relative changes in Tau, p-Tau, protein phosphatase 2A (PP2A), and glycogen synthase kinase 3ß (GSK-3ß) by Western blotting and enzyme-linked immunosorbent assay. At 40 nmol/L OA, the cell viability of the SH-SY5Y cells was significantly changed. We used different concentrations of ICA and IC to treat AD model cells and found that the effect of 2.5 µmol/L ICA and 1 µmol/L ICT was best after 48 H of treatment. After SH-SY5Y cell induction, the p-Tau levels were increased (P < 0.05); after the ICA/ICT treatment, the p-Tau and GSK-3ß levels were decreased (P < 0.05), although PP2A expression did not change (P > 0.05). We found that ICA and ICT exert an effect on AD model cells by decreasing the levels of GSK-3ß and p-Tau. The therapeutic effect of ICT is slightly better than that of ICA. Although these drugs were effective in the cell model, more studies are required to determine whether they are promising for the treatment and prevention of AD.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença de Alzheimer Limite: Humans Idioma: En Revista: Biotechnol Appl Biochem Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença de Alzheimer Limite: Humans Idioma: En Revista: Biotechnol Appl Biochem Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 2022 Tipo de documento: Article