Pretreatment of Body Fluid Specimens Using Hyaluronidase and Ultracentrifugation.
Lab Med
; 52(5): 469-476, 2021 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-33560339
ABSTRACT
OBJECTIVE:
Viscous body fluids present challenges during clinical laboratory testing. The present study was conducted to evaluate the effectiveness of hyaluronidase (HYAL) and ultracentrifugation (UC) pretreatment for a variety of body fluids before clinical chemistry testing.METHODS:
The following body fluids were evaluated biliary/hepatic, cerebrospinal, dialysate, drain, pancreatic, pericardial, peritoneal/ascites, pleural, synovial, and vitreous. Analytes assessed included amylase, total bilirubin, cancer antigen 19-9, carcinoembryonic antigen, cholesterol, chloride, creatinine, glucose, lactate dehydrogenase, lipase, potassium, rheumatoid factor, sodium, total protein, triglycerides, urea nitrogen, and uric acid.RESULTS:
Observed percentage differences between HYAL treated and untreated fluids were less than ±15% for all analytes investigated, with a small number showing statistical significance (P <.05). In addition, UC showed increased variability for limited body fluid/analyte combinations.CONCLUSION:
The HYAL treatment effectively reduced viscosity for body fluids. Validation of specimen pretreatment processes ensures acceptable analytical performance and the absence of unanticipated interferences.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Líquidos Corporais
Limite:
Humans
Idioma:
En
Revista:
Lab Med
Ano de publicação:
2021
Tipo de documento:
Article