Increased satellite cell apoptosis in vastus lateralis muscle after anterior cruciate ligament reconstruction.
J Rehabil Med
; 53(2): jrm00153, 2021 Feb 17.
Article
em En
| MEDLINE
| ID: mdl-33569607
ABSTRACT
OBJECTIVE:
Recovery of the quadriceps femoris muscle after anterior ligament reconstruction is im-paired. The aim of this study was to investigate satellite cell content and function of the vastus lateralis muscle after anterior ligament reconstruction.METHODS:
Biopsies were obtained from the vastus lateralis muscle of 16 recreational athletes immediately before and again 12 weeks after anterior ligament reconstruction. Total satellite cell number (Pax7+), activated (Pax7+/MyoD+), differentiating (Pax7-/MyoD+), and apoptotic (Pax7+/TUNEL+) satellite cells, myofibers expressing myosin heavy chain (MHC) I and II, and neonatal MHC (MHCneo) were determined immunohistochemically.RESULTS:
After anterior ligament reconstruction, the number of apoptotic satellite cells was significantly (p = 0.019) increased, concomitant with a significant (p < 0.001) decrease in total satellite cell number, with no change in activated and differentiating satellite cell number. MHCneo+ myofibers tended towards an increase.CONCLUSION:
Satellite cell apoptosis and the reduction in the satellite cell pool might provide an explanation for prolonged quadriceps muscle atrophy after anterior ligament reconstruction.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Satélites de Músculo Esquelético
/
Músculo Quadríceps
/
Reconstrução do Ligamento Cruzado Anterior
Limite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Rehabil Med
Assunto da revista:
REABILITACAO
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Alemanha