In vitro therapeutic evaluation of nanoliposome loaded with Xyloglucans polysaccharides from Tamarindus flower extract.

ABSTRACT

Nanoparticles are interesting area of research developed for several diagnostic and therapeutic applications. Tamarind flower extract is rich in Xyloglucan, a starch like polysaccharide which promotes proliferation and various application areas like drug-delivery technology. In recent years researchers are evaluating nanoliposome using in vitro and in vivo studies to discover their biomedical applications. Considering the importance and feasibility of nanoliposome, the present study is focused on synthesis of liposomes via biological method. The biological molecules of Tamarindus indica flower were used for the synthesis of nanoliposome. The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition. UV-Vis spectral analysis revealed the emission of peak at 232 nm. Further, the chemical characterization using FTIR revealed the presence of components in the functional group. EDX analysis exhibited the presence of O, Na, P and Cl, while DLS confirmed bilayer formation of xyloglucan and liposomes with uniform size (70-80 nm) and spherical shape. The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test. Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD). The synthesized nanoliposome evaluated against Gram negative and Gram positive bacteria showed potential antibacterial activity. TifeLiNPs demonstrated significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity. Overall, the present study exhibited the potential application of TifeLiNPs for biomedical purposes.