Soluble expression, purification, and secondary structure determination of human MESP1 transcription factor.

Haridhasapavalan, Krishna Kumar; Ranjan, Sujal Harsh; Bhattacharyya, Srirupa; Thummer, Rajkumar P

Haridhasapavalan, Krishna Kumar; Ranjan, Sujal Harsh; Bhattacharyya, Srirupa; Thummer, Rajkumar P.

Afiliação

Haridhasapavalan KK; Laboratory for Stem Cell Engineering and Regenerative Medicine, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
Ranjan SH; Laboratory for Stem Cell Engineering and Regenerative Medicine, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
Bhattacharyya S; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
Thummer RP; Laboratory for Stem Cell Engineering and Regenerative Medicine, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India. rthu@iitg.ac.in.

Appl Microbiol Biotechnol ; 105(6): 2363-2376, 2021 Mar.

Article em En | MEDLINE | ID: mdl-33651130

Assuntos

Escherichia coli; Fatores de Transcrição; Animais; Fatores de Transcrição Hélice-Alça-Hélice Básicos; Códon; Escherichia coli/genética; Humanos; Proteínas Recombinantes de Fusão/genética; Proteínas Recombinantes/genética

Palavras-chave

E. coli; MESP1; Protein expression and purification; Recombinant protein; Salt-dependent aggregation; Secondary structure

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Escherichia coli Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Appl Microbiol Biotechnol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Índia

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