Purification and Structural Characterization of Dendrobium officinale Polysaccharides and Its Activities.
Chem Biodivers
; 18(5): e2001023, 2021 May.
Article
em En
| MEDLINE
| ID: mdl-33721383
ABSTRACT
In this study, Dendrobium officinale polysaccharide (named DOPS-1) was isolated from the stems of Dendrobium officinale by hot-water extraction and purified by using Sephadex G-150 column chromatography. The structural characterization, antioxidant and cytotoxic activity were carried out. Based on the results of HPLC, GC, Congo red experiment, together with periodate oxidation, Smith degradation, SEM, FT-IR, and NMR spectral analysis, it expressed that DOPS-1 was largely composed of mannose, glucose and galacturonic acid in a molar ratio of 3.2 1.3 1. The molecular weight of DOPS-1 was 1530â
kDa and the main chain was composed of (1â4)-ß-D-Glcp, (1â4)-ß-D-Manp and 2-O-acetyl-(1â4)-ß-D-Manp. The measurement results of antioxidant activity showed that DOPS-1 had the strong scavenging activities on hydroxyl radicals, DPPH radicals and superoxide radicals and the high reducing ability inâ
vitro. Moreover, DOPS-1 was cytotoxic to all three human cancer cells of MDA-MB-231, A549 and HepG2.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Extratos Vegetais
/
Dendrobium
/
Antineoplásicos Fitogênicos
/
Antioxidantes
Limite:
Humans
Idioma:
En
Revista:
Chem Biodivers
Assunto da revista:
BIOQUIMICA
/
QUIMICA
Ano de publicação:
2021
Tipo de documento:
Article