Plasma PrPC and ADAM-10 as novel biomarkers for traumatic brain injury and concussion: a pilot study.
Brain Inj
; 35(6): 734-741, 2021 05 12.
Article
em En
| MEDLINE
| ID: mdl-33760683
ABSTRACT
BACKGROUND:
Cellular prion protein (PrPC) is a lipid raft protein abundant within CNS. It is regulated by a disintegrin and metalloproteinase domain containing protein 10 (ADAM10). PrPC has previously been implicated as a biomarker for TBI. ADAM10 has not been investigated as a TBI biomarker.OBJECTIVE:
We evaluated PrPC and ADAM10 as candidate biomarkers for TBI.METHODS:
We performed ELISA for ADAM10 and PrPC on plasma samples of patients with TBI admitted to Brigham and Women's Hospital. Plasma samples from 20 patients admitted for isolated TBI were acquired from a biobank with clinical information. Control plasma (37 samples) was acquired from a commercial source. GraphPad was used to conduct statistical analysis.RESULTS:
37 controls and 20 TBI samples were collected. Of the patients with TBI, eight were mild, three were moderate, and nine were severe. Both PrPC and ADAM10 were elevated in patients with TBI compared with control (p < .001). ADAM10 exhibited greater expression in patients with worse clinical grade. There was no significant association of either PrPC or ADAM10 with time after injury.CONCLUSIONS:
Our results indicate that PrPC and ADAM10 appear to be useful potential tools for screening of TBI. ADAM10 is closely associated with clinical grade.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Príons
/
Lesões Encefálicas Traumáticas
Limite:
Female
/
Humans
Idioma:
En
Revista:
Brain Inj
Assunto da revista:
CEREBRO
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Canadá