Dual effects of hypoxia on proliferation and osteogenic differentiation of mouse clonal mesenchymal stem cells.
Bioprocess Biosyst Eng
; 44(9): 1831-1839, 2021 Sep.
Article
em En
| MEDLINE
| ID: mdl-33821326
ABSTRACT
Mouse clonal mesenchymal stem cells (mc-MSCs) were cultured on a Cytodex 3 microcarrier in a spinner flask for a suspension culture under hypoxia condition to increase mass productivity. The hypoxia environment was established using 4.0 mM Na2SO3 with 10 µM or 100 µM CoCl2 for 24 h in a low glucose DMEM medium. As a result, the proliferation of mc-MSCs under hypoxic conditions was 1.56 times faster than the control group over 7 days. The gene expression of HIF-1a and VEGFA increased 4.62 fold and 2.07 fold, respectively. Furthermore, the gene expression of ALP, RUNX2, COL1A, and osteocalcin increased significantly by 9.55, 1.55, 2.29, and 2.53 times, respectively. In contrast, the expression of adipogenic differentiation markers, such as PPAR-γ and FABP4, decreased. These results show that the hypoxia environment produced by these chemicals in a suspension culture increases the proliferation of mc-MSCs and promotes the osteogenic differentiation of mc-MSCs.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteogênese
/
Antígenos de Diferenciação
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Diferenciação Celular
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Regulação da Expressão Gênica
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Proliferação de Células
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Células-Tronco Mesenquimais
Limite:
Animals
Idioma:
En
Revista:
Bioprocess Biosyst Eng
Assunto da revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Ano de publicação:
2021
Tipo de documento:
Article