Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralis.
Parasit Vectors
; 14(1): 198, 2021 Apr 12.
Article
em En
| MEDLINE
| ID: mdl-33845894
ABSTRACT
BACKGROUND:
The in vitro activity of the brown seaweed Dictyota spiralis against both Leishmania amazonensis and Trypanosoma cruzi was evaluated in a previous study. Processing by bio-guided fractionation resulted in the isolation of three active compounds, classified as diterpenes. In the present study, we performed several assays to detect clinical features associated to cell death in L. amazonensis and T. cruzi with the aim to elucidate the mechanism of action of these compounds on parasitic cells.METHODS:
The aims of the experiments were to detect and evaluate specific events involved in apoptosis-like cell death in the kinetoplastid, including DNA condensation, accumulation of reactive oxygen species and changes in ATP concentration, cell permeability and mitochondrial membrane potential, respectively, in treated cells.RESULTS:
The results demonstrated that the three isolated diterpenes could inhibit the tested parasites by inducing an apoptosis-like cell death.CONCLUSIONS:
These results encourage further investigation on the isolated compounds as potential drug candidates against both L. amazonensis and T. cruzi.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trypanosoma cruzi
/
Extratos Vegetais
/
Apoptose
/
Phaeophyceae
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Leishmania
/
Antiprotozoários
Idioma:
En
Revista:
Parasit Vectors
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Espanha