High-resolution targeted bisulfite sequencing reveals blood cell type-specific DNA methylation patterns in IL13 and ORMDL3.
Clin Epigenetics
; 13(1): 106, 2021 05 10.
Article
em En
| MEDLINE
| ID: mdl-33971943
ABSTRACT
BACKGROUND:
Methylation of DNA at CpG sites is an epigenetic modification and a potential modifier of disease risk, possibly mediating environmental effects. Currently, DNA methylation is commonly assessed using specific microarrays that sample methylation at a few % of all methylated sites.METHODS:
To understand if significant information on methylation can be added by a more comprehensive analysis of methylation, we set up a quantitative method, bisulfite oligonucleotide-selective sequencing (Bs-OS-seq), and compared the data with microarray-derived methylation data. We assessed methylation at two asthma-associated genes, IL13 and ORMDL3, in blood samples collected from children with and without asthma and fractionated white blood cell types from healthy adult controls.RESULTS:
Our results show that Bs-OS-seq can uncover vast amounts of methylation variation not detected by commonly used array methods. We found that high-density methylation information from even one gene can delineate the main white blood cell lineages.CONCLUSIONS:
We conclude that high-resolution methylation studies can yield clinically important information at selected specific loci missed by array-based methods, with potential implications for future studies of methylation-disease associations.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Interleucina-13
/
Metilação de DNA
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Epigênese Genética
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Sequenciamento de Nucleotídeos em Larga Escala
/
Epigenômica
/
Proteínas de Membrana
Tipo de estudo:
Clinical_trials
/
Observational_studies
/
Risk_factors_studies
Limite:
Adolescent
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Adult
/
Child
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Clin Epigenetics
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Suécia