TRUST4: immune repertoire reconstruction from bulk and single-cell RNA-seq data.
Nat Methods
; 18(6): 627-630, 2021 06.
Article
em En
| MEDLINE
| ID: mdl-33986545
ABSTRACT
We introduce the TRUST4 open-source algorithm for reconstruction of immune receptor repertoires in αß/γδ T cells and B cells from RNA-sequencing (RNA-seq) data. Compared with competing methods, TRUST4 supports both FASTQ and BAM format and is faster and more sensitive in assembling longer-even full-length-receptor repertoires. TRUST4 can also call repertoire sequences from single-cell RNA-seq (scRNA-seq) data without V(D)J enrichment, and is compatible with both SMART-seq and 5' 10x Genomics platforms.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Algoritmos
/
Receptores Imunológicos
/
Análise de Sequência de RNA
/
Análise de Célula Única
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos