Generation of sequence diversity in the kinetoplast DNA minicircles of Leishmania mexicana amazonensis.
Mol Biochem Parasitol
; 30(1): 1-8, 1988 Jul.
Article
em En
| MEDLINE
| ID: mdl-3398889
ABSTRACT
In order to understand the mechanisms which generate minicircle sequence diversity, we sequenced three minicircles belonging to the same or closely related sequence classes from the kinetoplast DNA of Leishmania mexicana amazonensis strains, PH8, Raimundo, and Josefa. Closely related minicircles from PH8 and Raimundo were unexpectedly found to differ at 11% of positions within the evolutionarily conserved region, but at only 3.9% of positions in the variable region. It thus appears that accumulation of point mutations will not account for the wide intra-strain and intra-subspecies divergence of the variable region. Comparison of more distantly related minicircles from PH8 and Josefa revealed only two short stretches of 70% homology within the variable region. These stretches of homology are not located in the same positions relative to the conserved regions in their respective minicircles. They may represent vestiges of recombinational events responsible for the rapid divergence of minicircle variable regions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Variação Genética
/
Leishmania mexicana
/
DNA Circular
/
DNA Mitocondrial
Limite:
Animals
País/Região como assunto:
Mexico
Idioma:
En
Revista:
Mol Biochem Parasitol
Ano de publicação:
1988
Tipo de documento:
Article