CXCR3 alleviates renal ischemiareperfusion injury via increase of Tregs.
Mol Med Rep
; 24(1)2021 07.
Article
em En
| MEDLINE
| ID: mdl-34080653
ABSTRACT
Increasing evidence has demonstrated that regulatory T cells (Tregs) suppress innate immunity, as well as protect the kidneys from ischemiareperfusion injury (IRI) and offer a potentially effective strategy to prevent or alleviate renal IRI. The present study explored whether CXC motif chemokine receptor 3 (CXCR3) alleviated renal IRI by increasing Tregs. Male C57BL/6J mice were divided into shamsurgery, IRI, CXCR3 overexpression (OECXCR3)+IRI, PC61+IRI and OECXCR3+PC61+IRI groups. Histopathological examination of the kidney was carried out using hematoxylineosin and Masson staining. The levels of serum creatinine (Scr) and blood urea nitrogen (BUN) were measured. Blood and kidney levels of IL6, TNFα, CC motif chemokine ligand (CCL)2 and IL10 were detected by ELISA and western blotting. The levels of superoxide dismutase (SOD), glutathione peroxidase (GSHPx) and malondialdehyde (MDA) in kidney tissues were also measured to assess oxidative stress. The population of Tregs in the kidney was assessed using flow cytometry. The results demonstrated that administration of OECXCR3 to IRI mice significantly decreased the levels of Scr, BUN, IL6, TNFα, CCL2 and MDA, increased the levels of IL10, SOD and GSHPx, and mitigated the morphologic injury and fibrosis induced by IR compared with the IRI group. In addition, administration of OECXCR3 induced significant reductions in the expression levels of fibrosisrelated markers, including fibronectin and type IV collagen, and increased the number of Tregs. These roles of OECXCR3 were significantly neutralized following deletion of Tregs with PC61 (antiCD25 antibody). Together, the present study demonstrated that injection of OECXCR3 lentiviral vectors into animal models can alleviate renal IRI by increasing the number of Tregs. The results may be a promising approach for the treatment of renal IRI.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão
/
Receptores CXCR3
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2021
Tipo de documento:
Article