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Physiological Changes and Differential Gene Expression of Tea Plants (Camellia sinensis (L.) Kuntze var. niaowangensis Q.H. Chen) Under Cold Stress.
Wang, Ying; Li, Yan; Wang, Jihong; Xiang, Zhun; Xi, Peiyu; Zhao, Degang.
Afiliação
  • Wang Y; The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-Bioengineering and College of Life Sciences, Guizhou University, Guiyang, China.
  • Li Y; Guizhou Province Institute of Biology, Guizhou Academy of Sciences, Guiyang, China.
  • Wang J; The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-Bioengineering and College of Life Sciences, Guizhou University, Guiyang, China.
  • Xiang Z; Guizhou Province Institute of Biology, Guizhou Academy of Sciences, Guiyang, China.
  • Xi P; Guizhou Province Institute of Biology, Guizhou Academy of Sciences, Guiyang, China.
  • Zhao D; Guizhou Province Institute of Biology, Guizhou Academy of Sciences, Guiyang, China.
DNA Cell Biol ; 40(7): 906-920, 2021 Jul.
Article em En | MEDLINE | ID: mdl-34129383
ABSTRACT
Low temperature is an important factor that affects the growth and reproduction of tea plants [Camellia sinensis (L.) Kuntze]. In this study, Yunwu Tribute Tea cutting seedlings [Camellia sinensis (L.) Kuntze var. niaowangensis Q.H. Chen] were subjected to different low-temperature treatments in Guizhou Province, China, and the changes in physiological indicators of the leaves were measured to investigate the physiological response and cold tolerance of this variety. Under cold stress, the peak of antioxidant enzyme activity appeared on the third day of treatment at 1°C, indicating that Yunwu Tribute Tea could improve the resistance to cold stress through an increase in enzyme activity within a low-temperature range. However, after 3 days treatment at 1°C, the tolerance of plant had been exceeded; the ability to resist cold stress disappeared, and enzyme activity decreased. When the temperature or duration of stress exceeded the maximum tolerance of the plant, the synthesis of soluble substances decreased in concert with their protective effects. Under cold conditions, Yunwu Tribute Tea could increase the production of abscisic acid growth inhibitors and reduce those of indoleacetic acid, gibberellin, and other growth promoting substances to manage cold stress by regulating the balance of growth regulators in the plant. Five differential genes were screened as candidate genes from the Yunwu Tribute Tea cold stress transcriptome (DW, 1°C) for fluorescence quantitative analysis. The results showed that the changes in levels of expression of these genes under continuous cold stress significantly positively correlated with the corresponding physiological indicators. Nevertheless, the levels of expression of the Yunwu Tribute Tea polyphenol oxidase (PPO) gene and the gibberellin 3ß-dioxygenase gene (G3O2) were reversely inhibited under cold stress. The result was consistent with the corresponding physiological indicators, and it provides a basis for the study of cold resistance mechanisms in tea plants.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Camellia sinensis / Resposta ao Choque Frio País/Região como assunto: Asia Idioma: En Revista: DNA Cell Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Camellia sinensis / Resposta ao Choque Frio País/Região como assunto: Asia Idioma: En Revista: DNA Cell Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China