Serological and molecular epidemiology of the Dengue, Zika and Chikungunya viruses in a risk area in Brazil.
BMC Infect Dis
; 21(1): 704, 2021 Jul 24.
Article
em En
| MEDLINE
| ID: mdl-34303348
ABSTRACT
BACKGROUND:
The co-circulation of types of arbovirus in areas where they are endemic increased the risk of outbreaks and limited the diagnostic methods available. Here, we analyze the epidemiological profile of DENV, CHIKV and ZIKV at the serological and molecular level in patients with suspected infection with these arboviruses in the city of Juazeiro do Norte, Ceará, Brazil.METHODS:
In 2016, the Central Public Health Laboratory (LACEN) of Juazeiro do Norte received 182 plasma samples from patients who visited health facilities with symptoms compatible with arbovirus infection. The LACEN performed serological tests for detection of IgM/IgG to DENV and CHIKV. They then sent these samples to the Retrovirology Laboratory of the Federal University of São Paulo and Faculty of Medical of the ABC where molecular analyses to confirm the infection by DENV, ZIKV and CHIKV were performed. The prevalence of IgM/IgG antibodies and of infections confirmed by RT-qPCR were presented with 95% confidence interval.RESULTS:
In serologic analysis, 125 samples were positive for antibodies against CHIKV and all were positive for antibodies against DENV. A higher prevalence of IgG against CHIKV (63.20% with 95% CI 45.76-70.56) than against DENV (95.05% with 95% CI 78.09-98.12) was observed. When the samples were submitted to analysis by RT-qPCR, we observed the following prevalence mono-infection by ZIKV of 19.23% (95% CI 14.29-34.82) patients, mono-infection by CHIKV of 3.84% (95% CI 2.01-5.44) and co-infection with ZIKV and CHIKV of 1.09% (95% CI 0.89-4.56).CONCLUSION:
The serologic and molecular tests performed in this study were effective in analyzing the epidemiological profile of DENV, CHIKV and ZIKV in patients with suspected infection by these arboviruses in the city of Juazeiro do Norte, Ceará/Brazil.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus Chikungunya
/
Dengue
/
Vírus da Dengue
/
Febre de Chikungunya
/
Zika virus
/
Infecção por Zika virus
/
Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
/
Etiology_studies
/
Observational_studies
/
Prevalence_studies
/
Risk_factors_studies
/
Screening_studies
Limite:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
BMC Infect Dis
Assunto da revista:
DOENCAS TRANSMISSIVEIS
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Brasil