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Multi-scale ensemble properties of the Escherichia coli RNA degradosome.
Dendooven, Tom; Paris, Giulia; Shkumatov, Alexander V; Islam, Md Saiful; Burt, Alister; Kubanska, Marta A; Yang, Tai Yuchen; Hardwick, Steven W; Luisi, Ben F.
Afiliação
  • Dendooven T; Department of Biochemistry, University of Cambridge, Cambridge, UK.
  • Paris G; Department of Biochemistry, University of Cambridge, Cambridge, UK.
  • Shkumatov AV; Center for Structural Biology, Vlaams Instituut voor Biotechnologie, Brussels, Belgium.
  • Islam MS; Structural Biology Brussels, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Brussels, Belgium.
  • Burt A; Department of Biochemistry, University of Cambridge, Cambridge, UK.
  • Kubanska MA; Institut de Biologie Structurale, Université Grenoble Alpes, CEA, CNRS, IBS, Grenoble, France.
  • Yang TY; Department of Biochemistry, University of Cambridge, Cambridge, UK.
  • Hardwick SW; Department of Biochemistry, University of Cambridge, Cambridge, UK.
  • Luisi BF; Department of Biochemistry, University of Cambridge, Cambridge, UK.
Mol Microbiol ; 117(1): 102-120, 2022 01.
Article em En | MEDLINE | ID: mdl-34415624
In organisms from all domains of life, multi-enzyme assemblies play central roles in defining transcript lifetimes and facilitating RNA-mediated regulation of gene expression. An assembly dedicated to such roles, known as the RNA degradosome, is found amongst bacteria from highly diverse lineages. About a fifth of the assembly mass of the degradosome of Escherichia coli and related species is predicted to be intrinsically disordered - a property that has been sustained for over a billion years of bacterial molecular history and stands in marked contrast to the high degree of sequence variation of that same region. Here, we characterize the conformational dynamics of the degradosome using a hybrid structural biology approach that combines solution scattering with ad hoc ensemble modelling, cryo-electron microscopy, and other biophysical methods. The E. coli degradosome can form punctate bodies in vivo that may facilitate its functional activities, and based on our results, we propose an electrostatic switch model to account for the propensity of the degradosome to undergo programmable puncta formation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polirribonucleotídeo Nucleotidiltransferase / RNA Bacteriano / Regulação Bacteriana da Expressão Gênica / RNA Helicases / Proteínas de Escherichia coli / Endorribonucleases / Escherichia coli / Complexos Multienzimáticos Tipo de estudo: Prognostic_studies Idioma: En Revista: Mol Microbiol Assunto da revista: BIOLOGIA MOLECULAR / MICROBIOLOGIA Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polirribonucleotídeo Nucleotidiltransferase / RNA Bacteriano / Regulação Bacteriana da Expressão Gênica / RNA Helicases / Proteínas de Escherichia coli / Endorribonucleases / Escherichia coli / Complexos Multienzimáticos Tipo de estudo: Prognostic_studies Idioma: En Revista: Mol Microbiol Assunto da revista: BIOLOGIA MOLECULAR / MICROBIOLOGIA Ano de publicação: 2022 Tipo de documento: Article