Multimodal high-resolution embryonic imaging with light sheet fluorescence microscopy and optical coherence tomography.
Opt Lett
; 46(17): 4180-4183, 2021 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-34469969
ABSTRACT
A high-resolution imaging system combining optical coherence tomography (OCT) and light sheet fluorescence microscopy (LSFM) was developed. LSFM confined the excitation to only the focal plane, removing the out of plane fluorescence. This enabled imaging a murine embryo with higher speed and specificity than traditional fluorescence microscopy. OCT gives information about the structure of the embryo from the same plane illuminated by LSFM. The co-planar OCT and LSFM instrument was capable of performing co-registered functional and structural imaging of mouse embryos simultaneously.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tomografia de Coerência Óptica
Limite:
Animals
Idioma:
En
Revista:
Opt Lett
Ano de publicação:
2021
Tipo de documento:
Article